bicinchoninic acid sds

SDS-PAGE and molecular cloning procedures were carried out by standard * This work was supported by National Institutes of Health Grants R29 DK46212 and RO1 AR44838 and grants from the Barnes-Jewish Hospital Foundation. xref The BCA Protein Assay is a detergent compatible formulation based on Bicinchoninic acid (BCA) for the colorimetric detection and quantitation of total protein. Protein determination is one of the most common operations performed in biochemical research. 23 0 obj<> endobj 0000000776 00000 n A bicinchoninic acid protein assay kit and glycerol were supplied by Fisher Scientific (UK). Home ... sodium dodecyl sulfate-polyacrylamide gel electrophoresis sds-page bioWORLD offers Bicinchoninic Acid Reagent for your research at low price. • Bicinchoninic acid disodium salt hydrate has been used to monitor the levels of reducing sugars, thereby helping in the detection of chitinases. bicinchoninic acid (see reference 1 on page 4). The Bicinchoninic Acid (BCA) Protein Assay is a highly sensitive colorimetric assay that is compatible with detergent solubilized protein solutions. 0000006069 00000 n ZERO BIAS - scores, article reviews, protocol conditions and more 0000011909 00000 n Click on the entry to view the PubMed entry . The principle of the BCA assay relies on the formation of a Cu2+ protein complex under alkaline conditions, followed by reduction of the Cu2+ to Cu+. Download SDS (MSDS) Region ... Bicinchoninic acid disodium salt (CAS 979-88-4) Product Citations See how others have used Bicinchoninic acid disodium salt (CAS 979-88-4). x�b```b``vc`e`��a�c@ >�(�ƞL}�|{�6��9�"`A�d�Q�cG��T�cM�~&�f:%�d��%�)500 MSDS# Validation Date In Case of Emergency Print Date Section 1. 2,2'-Bicinchoninic Acid ... Safety Data Sheet (SDS) Language. %%EOF In particular, it is insensitive to detergents such as Triton X-100 and SDS (1 %). Additionally, the stability 'Abbreviations used: BCA, bicinchoninic acid; SDS, sodium dodecyl sulfate; BSA, bovine serum albumin; VLDL, very low density lipoprotein; LDL, low density lipoprotein; HDL, high density lipoprotein. Specifications . The first step is the chelation of copper with protein in an alkaline environment to form a light blue complex. Chemical Product and Company Identification Responsible NamePierce Administration Synonyms Bicinchoninic Acid Protein Assay Reagent A Intended Use Refer to the instruction booklet for proper and intended use. Download SDS (MSDS) Region ... 2,2prime-Bicinchoninic Acid (CAS 1245-13-2) Product Citations See how others have used 2,2prime-Bicinchoninic Acid (CAS 1245-13-2). The first step is the chelation of copper with protein in an alkaline environment to form a light blue complex. 0000001086 00000 n Bioz Stars score: 99/100, based on 16 PubMed citations. C of A & Other Certificates. PRECISIO ® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution; Sigma-Aldrich pricing. Substances used for the detection, identification, analysis, etc. Immediate medical attention is required. Search. SDS was supplied by Melford (UK). The BCA Protein Assay combines the well-known reduction of Cu 2+ to Cu 1+ by protein in an alkaline medium with the highly sensitive and selective colorimetric detection of the cuprous cation (Cu 1+) by bicinchoninic acid (BCA). Search. Bicinchoninic acid (BCA) assay or Smith assay is a copper-based colorimetric assay for total protein quantification. The generated Cu2+ ion forms an intensely colored complex with the bicinchoninic acid reagent with a very strong absorbance band centered at 562 nm. Bicinchoninic acid is most commonly employed by biochemists in the bicinchoninic acid (BCA) assay, which is used to determine the total level of protein in a solution.In this assay, two molecules of bicinchoninic acid chelate a single Cu + ion, forming a purple water-soluble complex that strongly absorbs light at 562 nm. 0000001024 00000 n Search results for Bicinchoninic Acid (BCA) at Sigma-Aldrich The Bicinchoninic acid method combines the biuret reaction, i.e., the reduction of Cu2+ ions to Cu+ by proteins in an alkaline medium with complexation of the latter with bicinchoninic acid. DOWNLOAD MSDS (PDF) | Search MSDS currently available for download bioWORLD offers BCA (Bicinchoninic Acid) Protein Assay Kit with BSA Standards for your research at low price. Bicinchoninic acid is most commonly employed by biochemists in the bicinchoninic acid (BCA) assay, which is used to determine the total level of protein in a solution. 0000003471 00000 n First-aid measures General Advice Immediate medical attention is required. Bicinchoninic acid solution is used in the bicinchoninic acid (BCA) assay, which is equivalent to the Lowry procedure. The Bicinchoninic Acid (BCA) Protein Assay is a highly sensitive colorimetric assay that is compatible with detergent solubilized protein solutions. trailer 25 0 obj<>stream safety data sheet cat# k154-2500, -5000, bca protein assay kit ii sds date: jun 4, 2015 section 1: product and company identification . h޼�Qo7��"`/&��TE�ȳ$�L2�I6e��/d�kcK�,c��-�C;[��&y�f��,'g'�ԓ�l��v2��?N�v�dI�Ӳ�N�S=�'�}v�׋�#g'?Yj�=<>��vw}��nw�O��_��w��u��$ϧ�y�� F?�}�;=��}��on�>�|?�ɲD}�7^��7/{��m�{��[>D��30�$�A��=��߼x��I�M�M�M�M�M�M�M�M�!��s�9��=?��c��vhg3�`k�m�@�L��π��q`�Y`s9 *��Wss2zJ��!��9f��@`E. Bicinchoninic Acid, supplied by Millipore, used in various techniques. Stock for this item is limited, but may be available in a warehouse close to you. • It has also been used to determine the amount of cellulose reducing ends (micromoles/g cellulose). The amount of reduction is proportional to the protein … Please Contact Us for more information. Eye Contact Rinse immediately with plenty of water, also under the eyelids, for at least 15 minutes. Search results for Bicinchoninic Acid (BCA) at Sigma-Aldrich Specification Name Specification Value; Package Contents: BCA Solution, 2 x 250 ml, Copper Solution, 10 ml, Bovine Serum Albumin Standard, 2 mg/ml, 2 x 5 ml Y2 - Indicates an exempt polymer that is a polyester and is made only from reactants included in a specified list of low concern reactants product name: product codes: bca protein assay kit ii section 2: hazards identification The Bicinchoninic Acid (BCA) Protein Assay is a highly sensitive colorimetric assay that is compatible with detergent solubilized protein solutions. 0 %PDF-1.2 %�� The bicinchoninic acid protein assay is more sensitive than the Lowry method and less subject to interference. The purple-colored reaction product of this assay is formed by the chelation of two molecules of BCA with one cuprous ion. Type Lot Number Please enter Lot Number Incorrect Lot Number is entered. of reagents and the overall simplicity of the … endstream endobj 10547 0 obj <>stream Specifications . Search. Otherwise, contact supplier for specific applications. The intensity of the blue complex is proportional to the amount of protein in the sample. 0000000016 00000 n Specifications . 0000013632 00000 n Firstly, the peptide bonds in the protein sample reduce Cu 2+ ions, in a temperature dependent reaction, from the copper Bicinchoninic acid / baɪsɪnkɔːnɪnɪk / is a weak acid composed of two carboxylated quinoline rings. bicinchoninic acid (see reference 1 on page 4). The Bicinchoninic acid method is faster and easier than Lowry, with much greater tolerance to interference from non-ionic detergents and buffer salts. 0000005596 00000 n The Bicinchoninic Acid (BCA) Protein Assay primarily relies on two reactions. .. Although other methods, principally those based on the method of Lowry et al. Show this safety data sheet to the doctor in attendance. Bioz Stars score: 99/100, based on 1591 PubMed citations. The BCA Protein Assay combines the well-known reduction of Cu 2+ to Cu 1+ by protein in an alkaline medium with the highly sensitive and selective colorimetric detection of the cuprous cation (Cu 1+) by bicinchoninic acid (BCA). The purple-colored reaction product of this assay is formed by the chelation of two molecules of BCA with one cuprous ion. Application. [ �@k�� Ҁd*HÞ@Z�%�*�x�>�q`avH8��. ... Bicinchoninic Acid solution. 193, 265-275) have been extens … 0000014295 00000 n 0000004532 00000 n The Bicinchoninic Acid Assay (BCA), also referred to as the Smiths' Assay, is a biochemical assay designed by Paul Smith in 1985. (Smith, et al., [7]) This assay uses colorimetric detection and quantification to determine the total concentration of protein in a solution by exhibiting a measurable color change. Sodium polyanethol sulfonate (SPS) is an anionic detergent with a broad range of activities and applications. SDS; IRS1 (600-1245), GST tagged human. Techniques: Double Staining, TUNEL Assay, Staining, Expressing View product specific information, MSDS, References and Buying FAQ. Please Contact Us for more information. Chem. The BCA Protein Assay is a detergent compatible formulation based on Bicinchoninic acid (BCA) for the colorimetric detection and quantitation of total protein. 0000009016 00000 n 1 Product Result | Match Criteria: Product Name Synonym ... ≥95% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture; Sigma-Aldrich Keep eye wide open while rinsing. Click on the entry to view the PubMed entry . 0000005828 00000 n The requested SDS is not available. Detailed in this appendix are some commonly used methods for protein analysis, e.g., Lowry, Bradford, bicinchoninic acid (BCA), UV spectroscopic, and 3-(4-carboxybenzoyl)quinoline-2-carboxaldehyde (CBQCA) as … Description: Bicinchoninic acid disodium salt, Cas NO: 979-88-4, Molecular formula: C20H10N2O4Na2, ... SDS. Bicinchoninic Acid (BCA) Protein Assay Cat.# 786-570 Part # Description 030B BCA Solution (BCA Reagent A) 224B-B BSA (2mg/mL) Solution 274C Copper Solution (BCA Reagent B) Cat.# 786-571 Part # Description 030B BCA Solution (BCA Reagent A) 224B-B BSA (2mg/mL) Solution 274C Copper Solution (BCA Reagent B) Last saved: 7/30/2015 TN oe��V�KYm��}��ŧ �Z�% ~�׆olFn}��d��I�t)��%�a�S ��\��y��T� �¹V!��×v�M �F�ru�4��h�k|= �D��T��oD0௔K�!��2��6B�k��_�C��Z�ŀ��pT��P��h�v�Wj(H|�j\�_l�&��̽�J�w��$Z~\��4w�$�-m�qI4�K��e8��[ |�>5�[ ��"�(�`}��[|I��?%oˀ8E9�.�2+�q�ԩ�K,�6 ��i?��B �r�V隶��^��w��lL��}9L�r��". of chemical, biological, or pathologic processes or conditions. Type Lot Number Please enter Lot Number Incorrect Lot Number is entered. BCA rely on the formation of a Cu 2 + –protein complex in a basic environment, followed by reduction of the Cu 2 + to Cu + ( Smith et al., 1985 ). • … The costs of publication of this article were de- Biochemical analysis of proteins relies on accurate quantification of protein concentration. Introduction. View product specific information, MSDS, References and Buying FAQ. 0000006310 00000 n Trichloroacetic acid Revision Date 18-Jan-2018 Y1 - Indicates an exempt polymer that has a number-average molecular weight of 1,000 or greater. Falcon 96-well plates were supplied by BD Biosciences (USA). The requested SDS is not available. The principle of the BCA assay relies on the formation of a Cu2+ protein complex under alkaline conditions, followed by reduction of the Cu2+ to Cu+. Indicators are substances that change in physical appearance, e.g., color, at or approaching the endpoint of a chemical titration, e.g., on the passage between acidity and alkalinity. 0000002413 00000 n using the Bicinchoninic Acid (BCA) protein assay (Pierce). Download SDS (MSDS) Region ... Bicinchoninic acid disodium salt (CAS 979-88-4) Product Citations See how others have used Bicinchoninic acid disodium salt (CAS 979-88-4). C of A & Other Certificates. Inquire for Price. 0000014567 00000 n Please select Language. Trichloroacetic acid 76-03-9 >95 4. 0000014025 00000 n 23 24 C of A & Other Certificates. Firstly, the peptide bonds in the protein sample reduce Cu 2+ ions, in a temperature dependent reaction, from the copper Click on the entry to view the PubMed entry . The first step is the chelation of copper with protein in an alkaline environment to form a light blue complex. The Bicinchoninic Acid (BCA) Protein Assay primarily relies on two reactions. Bicinchoninic acid disodium salt hydrate. Search. While studying the excretion of cytoplasmic proteins in Staphylococcus aureus SPS was used as cell lysis inhibitor. ��6�f�A��vD �J��Q��Ѳ~��[�o ��\�i]��OX�r�"��Ƕ�h9�"i#�*�3;H��}pF�� t��ۖ�� 0000006559 00000 n 0000015256 00000 n 0000010084 00000 n ZERO BIAS - scores, article reviews, protocol conditions and more. The requested SDS is not available. Article Snippet: The One step TUNEL apoptosis assay kit, DAPI, RIPA lysis buffer, an SDS-PAGE kit and bicinchoninic acid (BCA) protein assay kit were purchased from Beyotime Institute of Biotechnology. 0000001359 00000 n View product specific information, MSDS, References and Buying FAQ. The Bicinchoninic Acid (BCA) Protein Assay primarily relies on two reactions. The bicinchoninic acid assay (BCA assay), also known as the Smith assay, after its inventor, Paul K. Smith at the Pierce Chemical Company, now part of Thermo Fisher Scientific, is a biochemical assay for determining the total concentration of protein in a solution (0.5 μg/mL to 1.5 mg/mL), similar to Lowry protein assay, Bradford protein assay or biuret reagent. startxref %��Z��p Type. Exoproteins from the indicated strains were quantified by the bicinchoninic acid assay, loaded in equal amounts, separated on 12% SDS-polyacrylamide gels, Techniques Used: Expressing, Acid Assay 6) Product Images from "CRISPR editing of mutant IDH1 R132H induces a CpG methylation-low state in patient-derived glioma models of G-CIMP" The bicinchoninic acid (BCA) assay method for the determination of protein has been investigated for its utility in measuring the protein content of plasma lipoproteins. <]>> Description. This water-soluble complex exhibits a strong absorbance at 562 nm that is nearly linear with increasing protein concentrations over a broad working range (20–2000 µg/mL). read more → BCA (Bicinchoninic Acid) Protein Assay primarily reduces Cu2+ to Cu1+ by proteins in an alkaline environment followed by highly sensitive and selective colorimetric detection of BCA/copper complex. Exoproteins from the indicated strains were quantified by the bicinchoninic acid assay, loaded in equal amounts, separated on 12% SDS-polyacrylamide gels, Techniques Used: Expressing, Acid Assay 6) Product Images from "CRISPR editing of mutant IDH1 R132H induces a CpG methylation-low state in patient-derived glioma models of G-CIMP" Eng Both rely on the formation of a Cu 2+-protein complex under alkaline conditions, followed by reduction of the Cu 2+ to Cu 1+. Thermo Fisher pierce bicinchoninic acid bca protein assay kit Pierce Bicinchoninic Acid Bca Protein Assay Kit, supplied by Thermo Fisher, used in various techniques. Please Contact Us for more information. This water-soluble complex exhibits a strong absorbance at 562 nm that is nearly linear with increasing protein concentrations over a broad working range (20–2000 µg/mL). Article Title: Heat Shock Protein 90 Has Roles in Intracellular Calcium Homeostasis, Protein Tyrosine Phosphorylation Regulation, and Progesterone-Responsive Sperm Function in Human Sperm Article Snippet: ..The concentrations of sperm protein extracts were quantified using the Bicinchoninic Acid Assay Kit (Beyotime Institution of Biotechnology, Haimen, China). Please make sure that you are logged in to the site so that available stock can be displayed. {�|u��/� ��C 0000007941 00000 n 0000010333 00000 n The BCA Protein Assay combines the well-known reduction of Cu 2+ to Cu 1+ by protein in an alkaline medium with the highly sensitive and selective colorimetric detection of the cuprous cation (Cu 1+) by bicinchoninic acid (BCA). 0000015290 00000 n (1951, J. Biol.

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